counting cell

By counting cell, determining OD after oil red 0 staining to investigate the effect of CLA on proliferation and differentiation of rat preadipocyte.

利用细胞计数，细胞形态观察和油红O染色OD值测定等方法研究了CLA对大鼠前体脂肪细胞增殖与分化的影响。CLA可减少脂肪细胞数目。

The characteristics of this method are: a, directly counting cell number without the influence of the metabolic state of the cells; b, discrimination of target cells from effector cells in cell-mediated cytotoxicity assay;

通过对该方法的验证，证明该方法具有：a，不受所测细胞代谢状态的影响：b，在细胞介导的细胞毒性试验中，能区分靶细胞和效应细胞；

The growth of cells was observed by counting cell number.

细胞计数法观察细胞的生长状况；

The new counting cell positions are used to generate additional pole counts.

计数单元的新位置就用来形成新补充的极点数.

Counting cell numbers on cultured 0 hour, 12,24 and 36 hours obtained cell growth curve;

计数0、12、24、36h的各组细胞数目并绘制细胞生长曲线；

Defect the reproductive ability of each group by counting cell clones, counting cells per clone and MTT chromatometry.

通过细胞克隆形成计数、单克隆细胞计数及MTT比色法等方法检测各组细胞的增殖能力。

Cell adhesion was evaluated by counting cell numbers at 0.5h, 2h and 4h. The MTT assay and the pNPP phosphatase assay were used for cell proliferation and differentiation measurement.

于0.5.2和4小时进行细胞计数，计算细胞吸附率。分别利用MTT法，pNPP磷酸酶法评价细胞的增殖和分化。